Principle: spin column (silica membrane)
Sample Size : up to 1.5 ml of fresh/ frozen blood; up to 6 x 10 of cultured cells
Column Capacity: 150 µg of DNA
Average DNA yield : 35 µg/ 1 ml whole blood
Handling Time: 1 hour
Elution Volume: 1 ml

Important Notes:
1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. Preheat a thermal incubator to 65 °C before the operation.
3. Use a centrifuge with a swinging bucket rotor and a force of 4,500 ~ 6,000 x g for in all centrifugation steps.
4. Add sterile ddH2O to proteniase K tube to make a 10 mg/ ml stock solution. Vortex and make sure that Proteinase K powder has been completely dissolved. Store the stock solution at 4 °C.
5. Add ethanol (96~100 %) to Wash W1 Buffer and Wash W2 Buffer before first use and store at room temperature.