Important Notes:
1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. Add 150 µl sterile ddH2O to lysozyme tube to make a 20 mg/ml stock solution. Vortex and make sure that lysozyme has been completely dissolved. Aliquot the lysozyme stock into small fractions and store the unused portions at -20 °C.
3. Add 100 µl sterile ddH2O to Proteinase K tube to make a 20 mg/ml stock solution. Vortex and make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 °C.
4. Add required volume ethanol (96- 100 %) to Wash Buffer W1 and W2 when first use.
5. Prepare two dry baths or two water baths before the operation: one to 37 °C for step 2 and the other to 60 °C for step 3.
6. Perheat the Elution Buffer or ddH2O for step 11 (Elution step). 7. All centrifuge steps are done at full speed (14,000 rpm or 18,000 x g) in a microcentrifuge.