Sample Size: up to 200 mg agarose gel slice
up to 100 μl PCR or other enzymatic reaction mixture 

Binding Capacity: up to 20 μg/ well
DNA Size range: 70 bp ~12Kb
Minimum elution volume: 50 µl
Operation: centrifuge/ vacuum manifold
Handling Time: about 40 minutes for gel DNA extraction about 30 minutes for PCR clean up
Recovery: 70~85 % for gel DNA extraction
90~95 % PCR clean up

Downstream application: Fluorescent or radioactive sequencing, Restriction digestion, Library screening, Ligation, Labeling, Transformation

Important Notes:
1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. The maxium sample size is described on specification, do not use the sample more than the limitation.
3. When excising the agarose gel, remove the extra gel to minimize the size of the gel.
4. Add ethanol (96~100 %) to Wash Buffer when first open.
5. Prepare bath to 55 °C before the operation for Gel DNA Extraction Protocol.
6. Preheat the Elution Buffer to 60 °C for DNA elution to increase the recovery of DNA fragment >1,000 bp.