Sample Amount: 500 mg (up to 1 g) plant tissue or 5~10 X 10 plant cells
Operation time: about 45~60 min
Binding Capacity: up to 1000 µg total RNA
Expected Yield: up to 50~300 µg total RNA from young leave
Elution volume: 500 µl 

Important Notes:
1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
3. Pipet 5 ml of FARB Buffer to another RNase-free container and add 50 µl of ß-mercaptoethanol (ß-ME) before every preparation.
4. Add required amount of ethanol (96-100%) Wash Buffer 2 when first open.
5. Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10mM MnCl2, 20mM Tris-HCl, pH7.0 at 25 °C) to final conc.= 0.5U/µl.