Principle: mini spin column (silica matrix)
Sample size: up to 100 mg plant tissue or 1 x10 plant cells
Operation time: 30 ~ 60 minutes
Binding capacity: up to 100 µg total RNA/ column
Expected yield: 5 ~30 µg of total RNA from 100 mg of young leave
Column applicability: centrifugation and vaccum
Minimum elution volume: 30 µl 

Important Notes:
1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
3. Pipet a required volume of FARB Buffer or FAPRB Buffer to another RNase-free container and add 10 μl ß-mercaptoethanol (ß-ME) per 1ml FARB Buffer or FAPRB Buffer before use. Caution: ß-mercaptoethanol is hazardous to human health. perform the procedures involving FARB Buffer or FAPRB Buffer in a chemical fume hood.
4. Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.
5. All centrifuge steps are done at full speed (~18,000 x g) in a microcentrifuge.
6. Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) to final conc. = 0.5 U/μl