Sample Amount: up to 100 mg plant tissue or 1 X 10 plant cells
Operation time: About 30~60 min
Binding Capacity: up to 100 µg total RNA
Expected Yield: up to 5~30 µg total RNA from young leave
Elution volume: 50 µl 

Important Notes:
1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
3. Pipet a required volume of FARB-1 Buffer to another RNase-free container and add 10 µl of ß-mercaptoethanol (ß-ME) per 1ml FARB-1 Buffer before use.
4. Add required volume of ethanol(96-100%) as bottle indicated to Wash Buffer 2 when first open.
5. Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10mM MnCl2, 20mM Tris-HCl, pH7.0 at 25 °C) to final conc.= 0.5U/µl.