Principle: mini spin column (silica matrix)
Operation time: 30 ~ 60 minutes
Binding capacity: up to 60 µg DNA/ column
Typical yield: 15 ~35 µg/ prep
Column applicability: centrifugation and vaccum
Minimum elution volume: 50 µl
Sample size: < 25 mg animal tissue
1.2 cm mouse tail
< 10 cultured cells

Important Notes:
1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Vortex and make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 °C.
3. Add ethanol (96- 100 %) to W1 Buffer and Wash Buffer when first open.
4. Prepare dry baths or water baths before the operation: one to 60 °C for step 4 and the other to 70 °C for step 7.
5. Preheat the Elution Buffer to 70 °C for step 13.
6. All centrifuge steps are done at full speed (~ 18,000 x g) in a microcentrifuge.