Principle: spin column (silica membrane)
Sample Size : up to 10 ml of fresh/ frozen blood; up to 1 x 10 of cultured cells
Column Capacity: 500 µg of DNA
Average DNA yield : 35 µg/ 1 ml whole blood
Handling Time: 1 hour
Elution Volume: 0.75~1.5 ml

Important Notes:
1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. Preheat a thermal incubator to 60 °C before the operation.
3. Use a centrifuge with a swinging bucket rotor and a force of 4,000 ~ 6,000 x g for in all centrifugation steps.
4. Add 500 µl of sterile ddH2O to proteniase K tube to make a 22 mg/ ml stock solution. Vortex and make sure that Proteinase K powder has been completely dissolved. Store the stock solution at 4 °C.
5. Perheat the Elution Buffer or ddH2O for step 11 (Elution step).