Principle: spin column (silica membrane)
Sample: up to 200 µl whole blood, serum, plasma, body fluids
Operation time: < 30 min
Principle: spin column (silica membrane)
Binding capacity: up to 60 µg/ column
DNA Yield: 4~8 µg/ 200 µl whole blood up to 5 x 10 cultured cells

Important Notes:
1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. Store proteinase K tube at -20 °C. Before first use, add required volume of sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Vortex and make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 °C.
3. Add required volume of ethanol (96- 100 %) to W1 Buffer and Wash Buffer when first use.
4. Preheat a dry bath or water bath to 60 °C before the operation.
5. All centrifuge steps are done at full speed (~18,000 x g) in a microcentrifuge.