Principle: mini spin column (silica matrix)
Sample size: 1 ~ 5 ml
Size of plasmid or construct: < 15 kb
Operation time: < 25 minutes
Typical Yield: 20 ~ 30 µg
Binding capacity: 60 µg/ column
Column applicability: centrifugation and vaccum 

Important Notes: 
1. Store RNase A at -20 °C upon recipit of kit. 
2. Add 0.5 ml of FAPD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the FAPD1 bottle, mix well by vortexing and store the FAPD1 buffer at 4 °C. 
3. If precipitates have formed in FAPD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates. 
4. Preparation of W1 Buffer and Wash Buffer by adding 96 ~100% ethanol (not provided) for first use. 
5. Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.